NP-101A, antifungal antibiotic from Streptomyces aurantiogriseus NPO-101.

In the course of our continuing search for antifungal antibiotics from microbial sources, we found an antifungal antibiotic against phytopathogenic fungi, especially Alternaria sp. from Actinomycete strain NPO-101. In the present report, we describe fermentation, isolation, physico-chemical properties, structure elucidation, and biological activities of NP-101A. The producing organism, strain NPO-101, was isolated from a soil sample collected in Sapporo, Hokkaido, Japan. The taxonomic characterization was carried out according to the methods used in the International Streptomyces Project (ISP)1}, the method of Pridham and Gottlieb2) and the method of Lechevalier3). The aerial myceliummass color was gray color series. Flexious spore chains were oval-shaped with a smooth surface. Melanoid pigments were produced and hydrolysis of whole-cell of this strain gave ll-DAP. The results of morphological and physiological studies